GSM4971429: Hepa.ChIPseq GR.H4.Dex.1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NR3C1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived hepatocytes
NA
NA
Attributes by original data submitter
Sample
source_name
Hepatocyte
cell type
hiPSC-derived HLCs
chip antibody
GR
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted by using Qiagen RNeasey Mini kit. DNA was isolated using phenol/chloroform extraction and NaCl/EtOH precipitated with 20 μg glycogen carrier. Transposed DNA was purified using a QIAGEN MinElute Reaction Cleanup kit. Transposed fragments were subjected to library preparation following manufacturer protocol The H3K4me3 in-situ ChIA-PET was performed according to the previously described protocol (Li et al., 2017) RNA libraries were prepared for sequencing by Novogene (CA). ChIP-seq library was prepared for sequencing according to the amplification protocol from Illumina. ATAC-seq libraries were sequenced using paired-end, dual-index sequencing on a NextSeq 500 instrument The H3K4me3 in-situ ChIA-PET was performed according to the previously described protocol (Li et al., 2017)